Topological model of LEAM within the inner mitochondrial membrane at low hydration. Model showing how LEAM in its alpha-helical form could insert within the inner leaflet of the inner mitochondrial membrane at low hydration, providing reinforcement of the membrane in the dry state. The helix would be stabilized by hydrophobic interactions with the fatty acid core of the membrane, and ionic interactions between positively charged residues (red) and negative charges of phospholipids. The negatively charged residues (blue) form a crest facing the matrix space. The scheme is inspired by the work of Tolleter et al. (2007). This indicates that seed mitochondria can efficiently oxidize exogenous NADH and produce ATP at negative temperature (Stupnikova et al., 2006).